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CO-LSO The LSO of rat stains intensely for cytochrome oxidase activity. This original image was digitally lightened for use in the banner above. Image credit E Bojman & A Cooper



double
                IF for VGLUT1 and Synaptotagmin 2
Immunofluorescence micrograph of rat auditory brainstem (coronal section), showing the expression of proteins VGLUT1 and Synaptotagmin 2. The LSO here measures about 500 µm left to right. See Cooper & Gillespie, 2011 for more information.


Pairs
Paired recording from synaptically connected MNTB and LSO principal cells. While recording from the LSO neuron (bottom), neurons in the MNTB are patched and stimulated (top). If the MNTB neuron provides input to the LSO cell, we see a post-synaptic current (red = average response). See Alamilla & Gillespie, 2011.



Prob_release
Paired-pulse responses for inhibitory (red) and excitatory (green) components of the MNTB input to the same LSO neuron. Paired-pulse depression, an indirect indicator of neurotransmitter release probability, is larger for the green component. This apparent difference in release probabilities suggests to us that whereas GABA, glycine, glutamate are co-released from the same terminals, they are released from different vesicles. See Case & Gillespie, 2011.



WebImage4 Higher magnification, double-immunofluroescence for the presynaptic proteins VGLUT1 (red) and Synaptotagmin 1 (green) in the LSO. Because red and green label virtually never colocalize, we infer that these proteins are targeted to different synaptic terminals. See Cooper & Gillespie, 2011.

WebImage5 By contrast, the presynaptic proteins VGLUT3 (red) and Synaptotagmin 1 (green) colocalize extensively (yellow) in the LSO, and we infer that these two proteins are in the same synaptic terminals. See Cooper & Gillespie, 2011.


kinetics
Post-synaptic receptors exhibit characteristic kinetics, depending on their subunit composition. At left are shown a glutamatergic response recorded from a young cell (green) and one from an older cell (blue). Developmental changes in the time-constant of exponential decay allow us to infer changes in receptor subunits with developmental age. See Case et al, 2011



LSO_cell
This cell in the LSO was visualized with a fluorescent dye (red), and the location of inhibitory inputs to the cell with immunofluorescence (yellow). Image credit A Cooper